In 1524, the former assassin Ezio Auditore, his wife Sophia and their children Marcelo and Flavia retired to a villa in the Tuscan countryside. When Ezio was writing in his study, Sophia asked him to visit his sister Claudia in Flavia and Marcelo. Not long after, he left his study to take care of their vineyard, while Flavia played near the villa. While harvesting, he saw a stranger dressed in black talking to Flavia. Due to worst-case concerns, Ezio hastily intercepted the stranger, despite Flavia telling him that she was not in danger because Ezio saw her necklace with an assassin's badge...